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pathscan phospho ikkβ sandwich elisa kit  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pathscan phospho ikkβ sandwich elisa kit
    Inhibition of <t>Ikkβ</t> phosphorylation at Ser177/181. Cell protein for the <t>ELISA</t> assay was obtained from THP-1 monocytes treated with 15 μM Lute and Kaem for 1 h, followed by LPS stimulation (10 μg/mL) for 30 min. Protein phosphorylation is expressed as the mean ± SD of the ratio between the absorbance (450 nm) and protein concentration (nm/mL). The experiment was repeated at least three times ( n = 3). ANOVA analysis and Holm–Sidak post hoc test allowed determination of statistically significant differences: * p < 0.05 versus LPS-stimulated THP-1 cells.
    Pathscan Phospho Ikkβ Sandwich Elisa Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ikk%CE%B2+ser177/PathScan+Phospho-IKKbeta+(Ser177%2F181)+Sandwich+ELISA+Kit/pmc13022854-125-12-19
    Average 93 stars, based on 29 article reviews
    pathscan phospho ikkβ sandwich elisa kit - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Buckwheat Flavonoids Modulate Inflammation in RAW 264.7 Macrophages at Physiologically Relevant Concentrations via the LPS/COX‑2 Pathway"

    Article Title: Buckwheat Flavonoids Modulate Inflammation in RAW 264.7 Macrophages at Physiologically Relevant Concentrations via the LPS/COX‑2 Pathway

    Journal: Journal of Agricultural and Food Chemistry

    doi: 10.1021/acs.jafc.6c02109

    Inhibition of Ikkβ phosphorylation at Ser177/181. Cell protein for the ELISA assay was obtained from THP-1 monocytes treated with 15 μM Lute and Kaem for 1 h, followed by LPS stimulation (10 μg/mL) for 30 min. Protein phosphorylation is expressed as the mean ± SD of the ratio between the absorbance (450 nm) and protein concentration (nm/mL). The experiment was repeated at least three times ( n = 3). ANOVA analysis and Holm–Sidak post hoc test allowed determination of statistically significant differences: * p < 0.05 versus LPS-stimulated THP-1 cells.
    Figure Legend Snippet: Inhibition of Ikkβ phosphorylation at Ser177/181. Cell protein for the ELISA assay was obtained from THP-1 monocytes treated with 15 μM Lute and Kaem for 1 h, followed by LPS stimulation (10 μg/mL) for 30 min. Protein phosphorylation is expressed as the mean ± SD of the ratio between the absorbance (450 nm) and protein concentration (nm/mL). The experiment was repeated at least three times ( n = 3). ANOVA analysis and Holm–Sidak post hoc test allowed determination of statistically significant differences: * p < 0.05 versus LPS-stimulated THP-1 cells.

    Techniques Used: Inhibition, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Protein Concentration

    Effect of BW flavonoids on the biosynthesis of TNF-α in LPS-stimulated RAW 264.7 macrophages. The culture medium obtained from cells stimulated with 100 ng/mL LPS in the presence of 15 μM Lute, Quer, Api, and Kaem for 4 h was analyzed by ELISA. The data are shown as mean ± SD from four independent replicates ( n = 4). ANOVA analyses followed by the Holm–Sidak post hoc test were used to determine statistically significant differences: ** p < 0.01 and *** p < 0.001 versus LPS-treated cells.
    Figure Legend Snippet: Effect of BW flavonoids on the biosynthesis of TNF-α in LPS-stimulated RAW 264.7 macrophages. The culture medium obtained from cells stimulated with 100 ng/mL LPS in the presence of 15 μM Lute, Quer, Api, and Kaem for 4 h was analyzed by ELISA. The data are shown as mean ± SD from four independent replicates ( n = 4). ANOVA analyses followed by the Holm–Sidak post hoc test were used to determine statistically significant differences: ** p < 0.01 and *** p < 0.001 versus LPS-treated cells.

    Techniques Used: Enzyme-linked Immunosorbent Assay



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    Image Search Results


    Inhibition of Ikkβ phosphorylation at Ser177/181. Cell protein for the ELISA assay was obtained from THP-1 monocytes treated with 15 μM Lute and Kaem for 1 h, followed by LPS stimulation (10 μg/mL) for 30 min. Protein phosphorylation is expressed as the mean ± SD of the ratio between the absorbance (450 nm) and protein concentration (nm/mL). The experiment was repeated at least three times ( n = 3). ANOVA analysis and Holm–Sidak post hoc test allowed determination of statistically significant differences: * p < 0.05 versus LPS-stimulated THP-1 cells.

    Journal: Journal of Agricultural and Food Chemistry

    Article Title: Buckwheat Flavonoids Modulate Inflammation in RAW 264.7 Macrophages at Physiologically Relevant Concentrations via the LPS/COX‑2 Pathway

    doi: 10.1021/acs.jafc.6c02109

    Figure Lengend Snippet: Inhibition of Ikkβ phosphorylation at Ser177/181. Cell protein for the ELISA assay was obtained from THP-1 monocytes treated with 15 μM Lute and Kaem for 1 h, followed by LPS stimulation (10 μg/mL) for 30 min. Protein phosphorylation is expressed as the mean ± SD of the ratio between the absorbance (450 nm) and protein concentration (nm/mL). The experiment was repeated at least three times ( n = 3). ANOVA analysis and Holm–Sidak post hoc test allowed determination of statistically significant differences: * p < 0.05 versus LPS-stimulated THP-1 cells.

    Article Snippet: Ikkβ phosphorylation at Ser177/181 was performed following the manufacturer’s instructions for a PathScan Phospho-Ikkβ sandwich ELISA kit (#7080) from Cell Signaling Technology (MA, USA).

    Techniques: Inhibition, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Protein Concentration

    Effect of BW flavonoids on the biosynthesis of TNF-α in LPS-stimulated RAW 264.7 macrophages. The culture medium obtained from cells stimulated with 100 ng/mL LPS in the presence of 15 μM Lute, Quer, Api, and Kaem for 4 h was analyzed by ELISA. The data are shown as mean ± SD from four independent replicates ( n = 4). ANOVA analyses followed by the Holm–Sidak post hoc test were used to determine statistically significant differences: ** p < 0.01 and *** p < 0.001 versus LPS-treated cells.

    Journal: Journal of Agricultural and Food Chemistry

    Article Title: Buckwheat Flavonoids Modulate Inflammation in RAW 264.7 Macrophages at Physiologically Relevant Concentrations via the LPS/COX‑2 Pathway

    doi: 10.1021/acs.jafc.6c02109

    Figure Lengend Snippet: Effect of BW flavonoids on the biosynthesis of TNF-α in LPS-stimulated RAW 264.7 macrophages. The culture medium obtained from cells stimulated with 100 ng/mL LPS in the presence of 15 μM Lute, Quer, Api, and Kaem for 4 h was analyzed by ELISA. The data are shown as mean ± SD from four independent replicates ( n = 4). ANOVA analyses followed by the Holm–Sidak post hoc test were used to determine statistically significant differences: ** p < 0.01 and *** p < 0.001 versus LPS-treated cells.

    Article Snippet: Ikkβ phosphorylation at Ser177/181 was performed following the manufacturer’s instructions for a PathScan Phospho-Ikkβ sandwich ELISA kit (#7080) from Cell Signaling Technology (MA, USA).

    Techniques: Enzyme-linked Immunosorbent Assay